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Mapping regulatory DNA that governs Drosophila eggshell patterning
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Pope, Nicole. Mapping regulatory DNA that governs Drosophila eggshell patterning. Retrieved from https://doi.org/doi:10.7282/T35Q4TQR

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TitleMapping regulatory DNA that governs Drosophila eggshell patterning
NamePope, Nicole (author); Yakoby, Nir (chair); Kotchoni, Simeon (internal member); Lee, Kwangwon (internal member); Rutgers University; Camden Graduate School
Date Created2013
Other Date2013-05 (degree)
SubjectBiology, Drosophila--Morphogenesis--Molecular aspects, Genetic markers, DNA
Extentvi, 42 p. : ill.
DescriptionOrganogenesis relies on extensive tissue patterning by regulating the expression of genes in a spatiotemporal manner. During oogenesis, the follicle cells, a monolayer of epithelial cells surrounding the developing oocyte, are patterned to drive the formation of the Drosophila eggshell; an organ that shelters the developing embryo. While follicle cells’ patterning has been vastly documented, the regulatory domains that govern tissue patterning are mostly unknown. To find regulatory domains, we cross-listed 81 genes known to be expressed during oogenesis with the large collection of the Gerald M. Rubin (GMR) lines containing noncoding DNA fragments, and we found 19 common genes. These genes are represented by 223 GMR lines. Of great advantage, all GMR lines are driving the expression of a GAL4 thus providing an opportunity to screen these lines by crossing them to a GFP reporter gene. Of the tested GMR lines, 55 lines, (25%), express GFP during oogenesis, and 18 lines (33%), recapitulate the partial or full endogenous pattern of their corresponding genes. We found that regulatory information is enriched in certain positions of the genes’ locus. We also demonstrated the use of the new driver lines to disrupt morphologies of the eggshell and other tissues. Our comprehensive screen identified multiple regulatory DNA fragments that govern eggshell patterning.
NoteM.S.
NoteIncludes bibliographical references
Noteby Nicole Pope
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T35Q4TQR
Languageeng
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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