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Iron responsive cell surface proteins in Thalassiosira pseudonana as probed by biotinylation and mass spectrometry
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New, Ashley M.. Iron responsive cell surface proteins in Thalassiosira pseudonana as probed by biotinylation and mass spectrometry. Retrieved from https://doi.org/doi:10.7282/T3HQ3XH1

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TitleIron responsive cell surface proteins in Thalassiosira pseudonana as probed by biotinylation and mass spectrometry
NameNew, Ashley M. (author); Kustka, Adam (chair); Hansen, Darren (internal member); Reinfelder, John (outside member); Rutgers University; Graduate School - Newark
Date Created2013
Other Date2013-05 (degree)
SubjectEnvironmental Science, Thalassiosira--Research, Thalassiosira--Effect of iron on, Mass spectrometry
Extentvi, 79 p. : ill.
DescriptionWe investigated the cell-surface enriched proteome of Thalassiosira pseudonana under growth rate limiting (60-70% ยต-max) and replete Fe conditions to better understand transporters that may be involved in Fe uptake. High and low Fe cultures were grown in the presence of 15N-NO3- (>98%) and 14N-NO3- (natural abundance), respectively, enabling relative quantification of proteins. In an effort to identify cell surface proteins, a cell surface labeling and enrichment method was developed and tested. Briefly, cell surface proteins were labeled with a free-amine reactive biotinylation reagent, soluble proteins were removed by membrane lysis and centrifugation, and biotinylated proteins were enriched on a neutravidin resin. Optimal conditions were sought for each of these three processes to increase coverage of cell surface labeled proteins. After elution, extracts were subjected to SDS-PAGE, in-gel tryptic digestion, and separation via liquid chromatography before identification and quantification by tandem mass spectrometry. Identification of cell surface proteins proved problematic due to biotinylation of some intracellular proteins and differential typtic digestion from the presence of the biotin linker arm. We obtained a greater than two-fold increase in abundance of the plasma membrane iron (III) permease (FTR1) under low Fe. A second FTR homolog was identified, indicating the presence of multiple Fe uptake pathways.
NoteM.S.
NoteIncludes bibliographical references
NoteIncludes vita
Noteby Ashley M. New
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3HQ3XH1
Languageeng
CollectionGraduate School - Newark Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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